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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM54 Recombinant antibody (83175-2-RR)

    WB analysis using 83175-2-RR (same clone as 83175-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using TRIM54 Recombinant antibody (83175-2-RR)

    IF Staining of C2C12 using 83175-2-RR (same clone as 83175-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM54 Recombinant antibody (83175-2-RR)

    IF Staining of HepG2 using 83175-2-RR (same clone as 83175-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using TRIM54 Recombinant antibody (83175-2-RR)

    FC experiment of MCF-7 using 83175-2-RR (same clone as 83175-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83175-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00236-3

    Cytometric bead array standard curve of MP00236-3

    Cytometric bead array standard curve of MP00236-3, TRIM54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83175-1-PBS. Detection antibody: 83175-2-PBS. Standard: Ag15229. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83175-2-RR

    Affinity and Kinetic Characterization of 83175-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

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TRIM54 Recombinant antibody, PBS Only (Detector)

TRIM54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83175-2-PBS
Clone No.230391G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83175-2-PBS targets TRIM54 as part of a matched antibody pair:

MP00236-3: 83175-1-PBS capture and 83175-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM54 fusion protein Ag15229 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 54
Calculated molecular weight 358 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC141807
Gene Symbol TRIM54
Gene ID (NCBI) 57159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYV2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).