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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM54 Recombinant antibody (83175-2-RR)

    WB analysis using 83175-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIM54 Recombinant antibody (83175-2-RR)

    IF Staining of HepG2 using 83175-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using TRIM54 Recombinant antibody (83175-2-RR)

    IF Staining of C2C12 using 83175-2-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using TRIM54 Recombinant antibody (83175-2-RR)

    FC experiment of MCF-7 using 83175-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83175-2-RR

    Affinity and Kinetic Characterization of 83175-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

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TRIM54 Recombinant antibody

TRIM54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83175-2-RR
Clone No.230391G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, mouse heart tissue
Positive IF/ICC detected inC2C12 cells, HepG2 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83175-2-RR targets TRIM54 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRIM54 fusion protein Ag15229 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 54
Calculated molecular weight 358 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC141807
Gene Symbol TRIM54
Gene ID (NCBI) 57159
RRIDAB_3670869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).

Protocols

Product Specific Protocols
WB protocol for TRIM54 antibody 83175-2-RRDownload protocol
IF protocol for TRIM54 antibody 83175-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols