TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-Ig

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53


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Tested Applications

Positive WB detected inHCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67403-1-Ig targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM72 fusion protein Ag2560 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 72
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033211
Gene symbol TRIM72
Gene ID (NCBI) 493829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM72 antibody 67403-1-IgDownload protocol
IF protocol for TRIM72 antibody 67403-1-IgDownload protocol
FC protocol for TRIM72 antibody 67403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols