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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM72 Monoclonal antibody

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-Ig

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis using 67403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of mouse skeletal muscle using 67403-1-Ig

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of mouse heart using 67403-1-Ig

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of pig heart using 67403-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    IF Staining of mouse skeletal muscle using 67403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    IF Staining of mouse skeletal muscle using 67403-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of C2C12 cells using TRIM72 Monoclonal antibody (67403-1-Ig)

    FC experiment of C2C12 using 67403-1-Ig

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHCT 116 cells, pig heart tissue, mouse skeletal muscle tissue, mouse heart tissue, pig skeletal muscle tissue
Positive IF-P detected inmouse skeletal muscle tissue
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67403-1-Ig targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM72 fusion protein Ag2560 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 72
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033211
Gene symbol TRIM72
Gene ID (NCBI) 493829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRIM72 antibody 67403-1-IgDownload protocol
IF protocol for TRIM72 antibody 67403-1-IgDownload protocol
FC protocol for TRIM72 antibody 67403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols