• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM72 Monoclonal antibody, PBS Only

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-PBS

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of mouse heart using 67403-1-Ig (same clone as 67403-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    WB analysis of pig heart using 67403-1-Ig (same clone as 67403-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using TRIM72 Monoclonal antibody (67403-1-Ig)

    IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using TRIM72 Monoclonal antibody (67403-1-Ig)

    FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67403-1-PBS targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM72 fusion protein Ag2560 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 72
Calculated molecular weight 477 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033211
Gene symbol TRIM72
Gene ID (NCBI) 493829
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.