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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using TRIP4 Polyclonal antibody (12324-1-AP)

    WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TRIP4 Polyclonal antibody (12324-1-AP)

    WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TRIP4 Polyclonal antibody (12324-1-AP)

    WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using TRIP4 Polyclonal antibody (12324-1-AP)

    WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using TRIP4 Polyclonal antibody (12324-1-AP)

    WB analysis of COLO 320 using 12324-1-AP (same clone as 12324-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIP4 Polyclonal antibody (12324-1-AP)

    IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIP4 Polyclonal antibody (12324-1-AP)

    IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIP4 Polyclonal antibody (12324-1-AP)

    IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TRIP4 Polyclonal antibody (12324-1-AP)

    IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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TRIP4 Polyclonal antibody, PBS Only

TRIP4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12324-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12324-1-PBS targets TRIP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIP4 fusion protein Ag2987 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor interactor 4
Calculated molecular weight 581 aa, 66 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC012448
Gene Symbol TRIP4
Gene ID (NCBI) 9325
RRIDAB_10646482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15650
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)