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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite® Plus 488-conjugated TRMT10C Polyclonal a (CL488-29087)

    IF Staining of U2OS using CL488-29087

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TRMT10C antibody (CL488-29087) at dilution of 1:200.

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CoraLite® Plus 488-conjugated TRMT10C Polyclonal antibody

TRMT10C Polyclonal Antibody for IF/ICC
Cat No. CL488-29087

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

RG9MTD1, MRPP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-29087 targets TRMT10C in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT10C fusion protein Ag30642 相同性解析による交差性が予測される生物種
Full Name RNA (guanine-9-) methyltransferase domain containing 1
Calculated molecular weight 47 kDa
Observed molecular weight40-45 kDa
GenBank accession numberNM_017819
Gene Symbol TRMT10C
Gene ID (NCBI) 54931
RRIDAB_3672836
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L0Y3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRMT10C antibody CL488-29087Download protocol
Standard Protocols
Click here to view our Standard Protocols