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  • Featured Product
  • KD/KO Validated

TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 18236-1-AP

Synonyms

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse brain using 18236-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

  • Western Blot (WB) analysis of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse lung using 18236-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of mouse lung using 18236-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using TRPC6 Polyclonal antibody (18236-1-AP)

    WB analysis of COLO 320 using 18236-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IP experiment of mouse lung using 18236-1-AP

    IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using TRPC6 Polyclonal antibody (18236-1-AP)

    IHC staining of human placenta using 18236-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TRPC6 Polyclonal antibody (18236-1-AP)

    IF Staining of A549 using 18236-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using TRPC6 Polyclonal antibody (18236-1-AP)

    FC experiment of A549 using 18236-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain, COLO 320 cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18236-1-AP targets TRPC6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC6 fusion protein Ag12976 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 6
Calculated molecular weight 931 aa, 106 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC093658
Gene symbol TRPC6
Gene ID (NCBI) 7225
RRIDAB_10859822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Protocols

Product Specific Protocols
WB protocol for TRPC6 antibody 18236-1-APDownload protocol
IHC protocol for TRPC6 antibody 18236-1-APDownload protocol
IF protocol for TRPC6 antibody 18236-1-APDownload protocol
IP protocol for TRPC6 antibody 18236-1-APDownload protocol
FC protocol for TRPC6 antibody 18236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Cell

Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.

Authors - Wei Rao
View Article
mouseWB,IF

Biomaterials

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Authors - Meitian Wang
View Article
humanIF

Proc Natl Acad Sci U S A

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.

Authors - Vladimir Riazanski
  • KO Validated
View Article
mouseWB

Phytomedicine

Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro

Authors - Zhongzhu Ai
View Article
mouseWB

Br J Pharmacol

Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.

Authors - Jiangnan Zhang
View Article
ratWB

Lab Invest

Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.

Authors - Xi He
View Article