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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using CoraLite® Plus 488-conjugated TRPC6 Polyclonal ant (CL488-18236)

    IF Staining of human placenta using CL488-18236

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 TRPC6 antibody (CL488-18236) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 488-conjugated TRPC6 Polyclonal ant (CL488-18236)

    FC experiment of A549 using CL488-18236

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody (CL488-18236)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRPC6 Polyclonal antibody

TRPC6 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-18236

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Synonyms

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman placenta tissue
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18236 targets TRPC6 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC6 fusion protein Ag12976 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 6
Calculated molecular weight 931 aa, 106 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC093658
Gene Symbol TRPC6
Gene ID (NCBI) 7225
RRIDAB_3084017
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y210
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
FC protocol for CL Plus 488 TRPC6 antibody CL488-18236Download protocol
Standard Protocols
Click here to view our Standard Protocols