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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPV1 Monoclonal antibody, PBS Only

TRPV1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3C9

Cat no : 66983-1-PBS

Synonyms

Capsaicin receptor, DKFZp434K0220, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of HepG2 using 66983-1-Ig (same clone as 66983-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-468 cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of MDA-MB-468 using 66983-1-Ig (same clone as 66983-1-PBS)

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of T-47D using 66983-1-Ig (same clone as 66983-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of HEK-293 using 66983-1-Ig (same clone as 66983-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of Y79 using 66983-1-Ig (same clone as 66983-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using TRPV1 Monoclonal antibody (66983-1-Ig)

    WB analysis of HUVEC using 66983-1-Ig (same clone as 66983-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66983-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66983-1-PBS targets TRPV1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRPV1 fusion protein Ag18683 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily V, member 1
Calculated molecular weight 839 aa, 95 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC132820
Gene symbol TRPV1
Gene ID (NCBI) 7442
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).