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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPV2 Recombinant antibody, PBS Only

TRPV2 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230141C3

Cat no : 83022-1-PBS

Synonyms

VRL, Vanilloid receptor-like protein 1, OTRPC2, Osm-9-like TRP channel 2, Osm 9 like TRP channel 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using TRPV2 Recombinant antibody (83022-1-RR)

    WB analysis of HT-1080 using 83022-1-RR (same clone as 83022-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 83022-1-RR (TRPV2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83022-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using TRPV2 Recombinant antibody (83022-1-RR)

    FC experiment of K562 using 83022-1-RR (same clone as 83022-1-PBS)

    1x10^6 K562 were intracellularly stained with 0.2 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83022-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83022-1-RR

    Affinity and Kinetic Characterization of 83022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83022-1-RR against Human TRPV2 were performed. The affinity constant is 31.3 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83022-1-PBS targets TRPV2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRPV2 fusion protein Ag8864 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily V, member 2
Calculated molecular weight 764 aa, 86 kDa
Observed molecular weight~80 kDa
GenBank accession numberBC018926
Gene symbol TRPV2
Gene ID (NCBI) 51393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transient receptor potential vanilloid 2 (TRPV2), also name as VRL-1, plays a critical role in neuronal development, cardiac function, immunity, and cancer (PMID: 31566564). It has been found to play an essential role in sustained intracellular Ca2+ concentration increase (PMID: 31857697). It's reported that TRPV2 is activated by high temperatures, with a threshold of around 52℃ (PMID: 10201375).