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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using human TRPV2 Recombinant antibody (83022-1-RR)

    WB analysis of HT-1080 using 83022-1-RR

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 83022-1-RR (TRPV2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K562 using TRPV2 Recombinant antibody (83022-1-RR)

    FC experiment of K562 using 83022-1-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using TRPV2 Recombinant antibody (83022-1-RR)

    FC experiment of SH-SY5Y using 83022-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83022-1-RR

    Affinity and Kinetic Characterization of 83022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83022-1-RR against Human TRPV2 were performed. The affinity constant is 31.3 pM.

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TRPV2 Recombinant antibody

TRPV2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83022-1-RR
Clone No.230141C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

VRL, Vanilloid receptor-like protein 1, OTRPC2, Osm-9-like TRP channel 2, Osm 9 like TRP channel 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells
Positive FC (Intra) detected inK562, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83022-1-RR targets TRPV2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRPV2 fusion protein Ag8864 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily V, member 2
Calculated molecular weight 764 aa, 86 kDa
Observed molecular weight~80 kDa
GenBank accession numberBC018926
Gene Symbol TRPV2
Gene ID (NCBI) 51393
RRIDAB_3670763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5S1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transient receptor potential vanilloid 2 (TRPV2), also name as VRL-1, plays a critical role in neuronal development, cardiac function, immunity, and cancer (PMID: 31566564). It has been found to play an essential role in sustained intracellular Ca2+ concentration increase (PMID: 31857697). It's reported that TRPV2 is activated by high temperatures, with a threshold of around 52℃ (PMID: 10201375).

Protocols

Product Specific Protocols
WB protocol for TRPV2 antibody 83022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols