TRPV4 Recombinant antibody
TRPV4 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241143H1
Cat no : 84091-4-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | unboiled rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 84091-4-RR is TRPV4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TRPV4 fusion protein Ag27253 相同性解析による交差性が予測される生物種 |
| Full Name | transient receptor potential cation channel, subfamily V, member 4 |
| Calculated molecular weight | 98 kDa |
| Observed molecular weight | 85~98 kDa |
| GenBank accession number | NM_001177428.1 |
| Gene symbol | TRPV4 |
| Gene ID (NCBI) | 59341 |
| RRID | AB_3671657 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV4 antibody 84091-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
