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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of A549 using 15314-1-AP (same clone as 15314-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of A549 using 15314-1-AP (same clone as 15314-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of SH-SY5Y using 15314-1-AP (same clone as 15314-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15314-1-PBS in a different storage buffer formulation.

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TSPAN14 Polyclonal antibody, PBS Only

TSPAN14 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 15314-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15314-1-PBS targets TSPAN14 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN14 fusion protein Ag7551 相同性解析による交差性が予測される生物種
Full Name tetraspanin 14
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC002920
Gene Symbol TSPAN14
Gene ID (NCBI) 81619
RRIDAB_2918039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG11
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TSPAN14 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN14 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).