TST Monoclonal antibody
TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2A6C5
Cat no : 66018-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 1 publications below |
Product Information
66018-1-Ig targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | TST fusion protein Ag9235 相同性解析による交差性が予測される生物種 |
Full Name | thiosulfate sulfurtransferase (rhodanese) |
Calculated molecular weight | 297 aa, 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC010148 |
Gene symbol | TST |
Gene ID (NCBI) | 7263 |
RRID | AB_11045660 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TST antibody 66018-1-Ig | Download protocol |
IHC protocol for TST antibody 66018-1-Ig | Download protocol |
IF protocol for TST antibody 66018-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfurtransfer kinetics. | ||
Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. | ||
J Biol Chem Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells. | ||
Biomolecules Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines. | ||
Mol Biol Rep Curcumin reduces myocardial ischemia-reperfusion injury, by increasing endogenous H2S levels and further modulating m6A | ||
Burns Cystathionine-gamma-lyase deficient mice are protected against the development of multiorgan failure and exhibit reduced inflammatory response during burn. |