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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TST Monoclonal antibody (66018-1-Ig)

    WB analysis using 66018-1-Ig (same clone as 66018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of HEK-293 using 66018-1-Ig (same clone as 66018-1-PBS)

    WB result of TST antibody (66018-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of mouse brain using 66018-1-Ig (same clone as 66018-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of mouse liver using 66018-1-Ig (same clone as 66018-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of rat liver using 66018-1-Ig (same clone as 66018-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of human brain using 66018-1-Ig (same clone as 66018-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of rat kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human liver cancer using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TST Monoclonal antibody (66018-1-Ig)

    IF Staining of A549 using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TST Monoclonal antibody, PBS Only

TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66018-1-PBS
Clone No.2A6C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2A6C5, EC:2.8.1.1, RDS, Rhodanese

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66018-1-PBS targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TST fusion protein Ag9235 相同性解析による交差性が予測される生物種
Full Name thiosulfate sulfurtransferase (rhodanese)
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010148
Gene Symbol TST
Gene ID (NCBI) 7263
RRIDAB_11045660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.