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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTC30A Monoclonal antibody, PBS Only

TTC30A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11B3

Cat no : 68232-1-PBS

Synonyms

FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC30A Monoclonal antibody (68232-1-Ig)

    WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TTC30A Monoclonal antibody (68232-1-Ig)

    WB analysis using 68232-1-Ig (same clone as 68232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC30A Monoclonal antibody (68232-1-Ig)

    IF Staining of hTERT-RPE1 using 68232-1-Ig (same clone as 68232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (68232-1-Ig, Clone: 1A11B3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, green). This data was developed using the same antibody clone with 68232-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68232-1-PBS targets TTC30A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TTC30A fusion protein Ag18361 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 30A
Calculated molecular weight 665 aa, 76 kDa
Observed molecular weight 72-76 kDa
GenBank accession numberBC042848
Gene symbol TTC30A
Gene ID (NCBI) 92104
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.