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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTK Polyclonal antibody (10381-1-AP)

    WB analysis using 10381-1-AP (same clone as 10381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using TTK Polyclonal antibody (10381-1-AP)

    WB analysis of MCF-7 using 10381-1-AP (same clone as 10381-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TTK Polyclonal antibody (10381-1-AP)

    WB analysis of HEK-293 using 10381-1-AP (same clone as 10381-1-PBS)

    WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of DU 145 cells using TTK Polyclonal antibody (10381-1-AP)

    IP experiment of DU 145 using 10381-1-AP (same clone as 10381-1-PBS)

    IP result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:600) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human breast cancer using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human testis using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human testis using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human kidney using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human kidney using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human liver cancer using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTK Polyclonal antibody (10381-1-AP)

    IHC staining of human liver cancer using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTK Polyclonal antibody (10381-1-AP)

    IF Staining of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using TTK Polyclonal antibody (10381-1-AP)

    FC experiment of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10381-1-PBS targets TTK in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTK fusion protein Ag0405 相同性解析による交差性が予測される生物種
Full Name TTK protein kinase
Calculated molecular weight 97 kDa
Observed molecular weight 96-97 kDa
GenBank accession numberBC000633
Gene Symbol TTK
Gene ID (NCBI) 7272
RRIDAB_2211979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33981
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).