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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated TWIST2 Monoclonal antibody (CL594-66544)

    IF Staining of HepG2 using CL594-66544

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TWIST2 antibody (CL594-66544, Clone: 1B12D11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated TWIST2 Monoclonal antibody (CL594-66544)

    FC experiment of HeLa using CL594-66544

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TWIST2 (CL594-66544, Clone:1B12D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66544
Clone No.1B12D11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66544 targets TWIST2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TWIST2 fusion protein Ag2348 相同性解析による交差性が予測される生物種
Full Name twist homolog 2 (Drosophila)
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
RRIDAB_3673504
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Protocols

Product Specific Protocols
IF protocol for CL594 TWIST2 antibody CL594-66544Download protocol
FC protocol for CL594 TWIST2 antibody CL594-66544Download protocol
Standard Protocols
Click here to view our Standard Protocols