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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated TXNRD1 Monoclonal antibody

TXNRD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B10C4

Cat no : CL594-67728

Synonyms

TRXR1, GRIM 12, Gene associated with retinoic and interferon-induced mortality 12 protein, Gene associated with retinoic and IFN-induced mortality 12 protein, EC:1.8.1.9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TXNRD1 Monoclonal antibody (CL594-67728)

    IF Staining of HeLa using CL594-67728

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 TXNRD1 antibody (CL594-67728, Clone: 1B10C4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated TXNRD1 Monoclonal antibody (CL594-67728)

    FC experiment of HeLa using CL594-67728

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TXNRD1 (CL594-67728, Clone:1B10C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-67728 targets TXNRD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TXNRD1 fusion protein Ag30071 相同性解析による交差性が予測される生物種
Full Name thioredoxin reductase 1
Calculated molecular weight 55 kDa
Observed molecular weight50-70 kDa
GenBank accession numberBC018122
Gene symbol TXNRD1
Gene ID (NCBI) 7296
RRIDAB_2920175
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

Protocols

Product Specific Protocols
IF protocol for CL594 TXNRD1 antibody CL594-67728Download protocol
FC protocol for CL594 TXNRD1 antibody CL594-67728Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Chem Biol Interact

Therapeutic Targeting of Thioredoxin Reductase 1 Causes Ferroptosis while Potentiating Anti-PD-1 Efficacy in Head and Neck Cancer

Authors - Ming-Shou Hsieh
View Article