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  • Featured Product
  • KD/KO Validated

HRP-conjugated TYMS Monoclonal antibody

TYMS Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

HRP

CloneNo.

1F5B7

Cat no : HRP-66725

Synonyms

HsT422, Thymidylate synthase, thymidylate synthetase, TMS, TS, TSase, TYMS

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HRP-conjugated TYMS Monoclonal antibody (HRP-66725)

    WB analysis of Jurkat using HRP-66725

    Jurkat cells were subjected to SDS PAGE followed by western blot with HRP-66725 (TYMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-66725 targets TYMS in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TYMS fusion protein Ag7027 相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002567
Gene symbol TYMS
Gene ID (NCBI) 7298
RRIDAB_2883845
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
WB protocol for HRP TYMS antibody HRP-66725Download protocol
Standard Protocols
Click here to view our Standard Protocols