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  • Featured Product
  • KD/KO Validated

Talin-1 Recombinant antibody

Talin-1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240294G5

Cat no : 82856-18-RR

Synonyms

TLN, talin1, talin 1, KIAA1027, ILWEQ

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Talin-1 Recombinant antibody (82856-18-RR)

    WB analysis of HeLa using 82856-18-RR

    WB result of Talin-1 antibody (82856-18-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using Talin-1 Recombinant antibody (82856-18-RR)

    WB analysis using 82856-18-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82856-18-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Talin-1 Recombinant antibody (82856-18-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Talin-1 Recombinant antibody (82856-18-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82856-18-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82856-18-RR (Talin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-18-RR)

    IF Staining of A549 using 82856-18-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-18-RR)

    IF Staining of A549 using 82856-18-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Recombinant antibody (82856-18-RR)

    IF Staining of A549 using 82856-18-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-18-RR, Clone: 240294G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red).

  • Flow cytometry (FC) experiment of HeLa cells using Talin-1 Recombinant antibody (82856-18-RR)

    FC experiment of HeLa using 82856-18-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Tln1 Recombinant Antibody (82856-18-RR, Clone:240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using Talin-1 Recombinant antibody (82856-18-RR)

    FC experiment of A549 using 82856-18-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Talin-1 Recombinant Antibody (82856-18-RR, Clone: 240294G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82856-18-RR

    Affinity and Kinetic Characterization of 82856-18-RR

    Biolayer interferometry (BLl) kinetic assays of 82856-18-RR against Human TLN1 were performed. The affinity constant is 0.923 nM.

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Tested Applications

Positive WB detected inPC-12 cells, HeLa cells, C6 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82856-18-RR is Talin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight~270 kDa
GenBank accession numberBC042923
Gene symbol Talin-1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
WB protocol for Talin-1 antibody 82856-18-RRDownload protocol
IHC protocol for Talin-1 antibody 82856-18-RRDownload protocol
IF protocol for Talin-1 antibody 82856-18-RRDownload protocol
FC protocol for Talin-1 antibody 82856-18-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols