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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Talin-1 Recombinant antibody

Talin-1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240294G5

Cat no : CL488-82856-18

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated Talin-1 Recombinant (CL488-82856-18)

    IF Staining of A549 using CL488-82856-18

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Talin-1 antibody (CL488-82856-18, Clone: 240294G5 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-82856-18 is Talin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight~270 kDa
GenBank accession numberBC042923
Gene symbol Talin-1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Talin-1 antibody CL488-82856-18Download protocol
Standard Protocols
Click here to view our Standard Protocols