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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E12D10

Cat no : CL750-67992

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 750-conjugated Talin-1 Monoclonal a (CL750-67992)

    FC experiment of HeLa using CL750-67992

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human Talin-1 (CL750-67992, Clone:2E12D10) (red), or 0.8 ug CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21) (CL750-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-67992 targets Talin-1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Talin-1 fusion protein Ag5937 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight 270 kDa
GenBank accession numberBC042923
Gene symbol Talin-1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 Talin-1 antibody CL750-67992Download protocol
Standard Protocols
Click here to view our Standard Protocols