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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tim23 Recombinant antibody (82540-1-RR)

    WB analysis using 82540-1-RR (same clone as 82540-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82540-1-RR (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Tim23 Recombinant antibody (82540-1-RR)

    IP experiment of HeLa using 82540-1-RR (same clone as 82540-1-PBS)

    IP result of anti-Tim23 (IP:82540-1-RR, 4ug; Detection:82540-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim23 Recombinant antibody (82540-1-RR)

    IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim23 Recombinant antibody (82540-1-RR)

    IHC staining of mouse heart using 82540-1-RR (same clone as 82540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Tim23 Recombinant antibody (82540-1-RR)

    IF Staining of HepG2 using 82540-1-RR (same clone as 82540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (82540-1-RR, Clone: 1H16 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82540-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82540-1-RR

    Affinity and Kinetic Characterization of 82540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82540-1-RR against Human Tim23 were performed. The affinity constant is below 1 pM.

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Tim23 Recombinant antibody, PBS Only

Tim23 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82540-1-PBS
Clone No.1H16

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TIMM23, TIMM 23, Mitochondrial import inner membrane translocase subunit Tim23, 1H16

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82540-1-PBS targets Tim23 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Tim23 fusion protein Ag1620 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC062707
Gene Symbol Tim23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14925
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)