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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Tim23 Recombinant antibody

Tim23 Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1H16

Cat no : 82540-1-RR

Synonyms

TIMM23, TIMM 23, Mitochondrial import inner membrane translocase subunit Tim23, 1H16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Tim23 Recombinant antibody (82540-1-RR)

    WB analysis using 82540-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82540-1-RR (Tim23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Tim23 Recombinant antibody (82540-1-RR)

    IP experiment of HeLa using 82540-1-RR

    IP result of anti-Tim23 (IP:82540-1-RR, 4ug; Detection:82540-1-RR 1:1000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim23 Recombinant antibody (82540-1-RR)

    IHC staining of mouse heart using 82540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Tim23 Recombinant antibody (82540-1-RR)

    IHC staining of mouse heart using 82540-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82540-1-RR (Tim23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Tim23 Recombinant antibody (82540-1-RR)

    IF Staining of HepG2 using 82540-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tim23 antibody (82540-1-RR, Clone: 1H16 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82540-1-RR

    Affinity and Kinetic Characterization of 82540-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82540-1-RR against Human Tim23 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82540-1-RR targets Tim23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Tim23 fusion protein Ag1620 相同性解析による交差性が予測される生物種
Full Name translocase of inner mitochondrial membrane 23 homolog (yeast)
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC062707
Gene symbol Tim23
Gene ID (NCBI) 10431
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tim23, a key component of the mitochondrial preprotein translocase, is anchored in the inner membrane by its C-terminal domain and exposes an intermediate domain in the intermembrane space that functions as a presequence receptor. Tim23 is the main component of the translocation channel of the TIM23 complex. (PMID: 10830167, PMID: 25083920)

Protocols

Product Specific Protocols
WB protocol for Tim23 antibody 82540-1-RRDownload protocol
IHC protocol for Tim23 antibody 82540-1-RRDownload protocol
IF protocol for Tim23 antibody 82540-1-RRDownload protocol
IP protocol for Tim23 antibody 82540-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols