CoraLite® Plus 488-conjugated Tim23 Monoclonal antibody
Tim23 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3H10F5
Cat no : CL488-67535
Synonyms
Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67535 targets Tim23 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Tim23 fusion protein Ag27069 相同性解析による交差性が予測される生物種 |
| Full Name | translocase of inner mitochondrial membrane 23 homolog (yeast) |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC062707 |
| Gene symbol | Tim23 |
| Gene ID (NCBI) | 10431 |
| RRID | AB_2919495 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Tim23 antibody CL488-67535 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
