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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Transketolase Monoclonal antibody, PBS Only

Transketolase Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E2F3

Cat no : 66016-1-PBS

Synonyms

TK, TKT, TKT1, Transketolase

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of Jurkat using 66016-1-Ig (same clone as 66016-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HeLa using 66016-1-Ig (same clone as 66016-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66016-1-Ig(Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66016-1-PBS targets Transketolase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779 相同性解析による交差性が予測される生物種
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.