• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis using 66016-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HeLa using 66016-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HepG2 using 66016-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human liver using 66016-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of Jurkat using 66016-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human brain using 66016-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human colon cancer using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human colon cancer using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    IF Staining of HepG2 using 66016-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66016-1-Ig
Clone No.3E2F3
Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue, Jurkat cells, human brain tissue, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66016-1-Ig targets Transketolase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779 相同性解析による交差性が予測される生物種
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene Symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
WB protocol for Transketolase antibody 66016-1-IgDownload protocol
IHC protocol for Transketolase antibody 66016-1-IgDownload protocol
IF protocol for Transketolase antibody 66016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun
View Article
humanWB

FASEB J

Prolactin receptor potentiates chemotherapy through miRNAs-induced G6PD/TKT inhibition in pancreatic cancer

Authors - Ying-Na Liao
View Article