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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E2F3

Cat no : CL488-66016

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Transketolase Monocl (CL488-66016)

    IF Staining of HepG2 using CL488-66016

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Transketolase antibody (CL488-66016, Clone: 3E2F3 ) at dilution of 1:600, CL594-phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66016 targets Transketolase in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779 相同性解析による交差性が予測される生物種
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_2883228
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Transketolase antibody CL488-66016Download protocol
Standard Protocols
Click here to view our Standard Protocols