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Species Reactivity

Species Reactivity

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Tubulin Beta Class V Monoclonal antibody, PBS Only

Tubulin Beta Class V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, Canine

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7B9

Cat no : 66362-1-PBS

Synonyms

HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    WB analysis of HeLa using 66362-1-Ig (same clone as 66362-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    WB analysis of human testis using 66362-1-Ig (same clone as 66362-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    WB analysis of NCCIT using 66362-1-Ig (same clone as 66362-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    WB analysis of NIH/3T3 using 66362-1-Ig (same clone as 66362-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    IHC staining of human lung cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    IHC staining of human breast cancer using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66362-1-Ig (Tubulin beta class V Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Tubulin Beta Class V Monoclonal antibody (66362-1-Ig)

    IF Staining of MDCK using 66362-1-Ig (same clone as 66362-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed MDCK cells using 66362-1-Ig(Tubulin beta class V antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66362-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66362-1-PBS targets Tubulin Beta Class V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tubulin, beta 6
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_032525
Gene symbol Beta Tubulin
Gene ID (NCBI) 84617
RRIDAB_2881742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.