Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
Product Information
66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, Canine samples.
| Tested Reactivity | human, mouse, Canine |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tubulin, beta 6 |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | NM_032525 |
| Gene Symbol | Beta Tubulin |
| Gene ID (NCBI) | 84617 |
| RRID | AB_2881742 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Tubulin Beta Class V antibody 66362-1-Ig | Download protocol |
| IHC protocol for Tubulin Beta Class V antibody 66362-1-Ig | Download protocol |
| IF protocol for Tubulin Beta Class V antibody 66362-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mediators Inflamm LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling | ||
Life Sci NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes. | ||
Dev Neurobiol Alpha- and beta-tubulin isotypes are differentially expressed during brain development. | ||
BMC Genomics Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats | ||