ChromoTek TurboGFP-Trap Agarose beads, kit
TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.
Specificity
TurboGFP, CopGFP, maxGFP
Applications
IP, CoIP
Type
Nanobody
Conjugate
Agarose
Cat no : tbtak
Synonyms
Validation Data Gallery
Product Information
TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.
| Description | The TurboGFP-Trap Agarose Kit contains TurboGFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of TurboGFP-fusion proteins and their interacting factors.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP |
| Specificity/Target | TurboGFP, CopGFP, maxGFP, maxFP-Green For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.1 mM citrate acid pH 3.0 |
| Wash buffer compatibility | 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827596 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| TurboGFP-Trap Agarose beads | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
Documentation
| SDS |
|---|
| SDS TurboGFP-Trap Agarose Kit (PDF) |
| Protocol |
|---|
| Protocol TurboGFP-Trap Agarose Kit (PDF) |
| Specificity |
|---|
| Fluorescent protein specificity table (PDF) |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
Publications
| Application | Title |
|---|---|
Cell Death Discov Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells |