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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    WB analysis using 68166-1-Ig (same clone as 68166-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    WB analysis of 4T1 using 68166-1-Ig (same clone as 68166-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of mouse liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of rat liver using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    FC experiment of HEK-293 using 68166-1-Ig (same clone as 68166-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68166-1-PBS in a different storage buffer formulation.

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U2AF2/U2AF65 Monoclonal antibody, PBS Only

U2AF2/U2AF65 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68166-1-PBS
Clone No.1B7E7

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68166-1-PBS targets U2AF2/U2AF65 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen U2AF2/U2AF65 fusion protein Ag8070 相同性解析による交差性が予測される生物種
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC008740
Gene Symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26368
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.