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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

U2AF2/U2AF65 Monoclonal antibody

U2AF2/U2AF65 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B7E7

Cat no : 68166-1-Ig

Synonyms

U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    WB analysis using 68166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of 4T1 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    WB analysis of 4T1 using 68166-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68166-1-Ig (U2AF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of mouse liver using 68166-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of mouse liver using 68166-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IHC staining of rat liver using 68166-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 68166-1-Ig (U2AF2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    IF Staining of HEK-293 using 68166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using U2AF2 antibody (68166-1-Ig, Clone: 1B7E7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using U2AF2/U2AF65 Monoclonal antibody (68166-1-Ig)

    FC experiment of HEK-293 using 68166-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human U2AF2 (68166-1-Ig, Clone:1B7E7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inLNCaP cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurket cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68166-1-Ig targets U2AF2/U2AF65 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen U2AF2/U2AF65 fusion protein Ag8070 相同性解析による交差性が予測される生物種
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit, is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protocols

Product Specific Protocols
WB protocol for U2AF2/U2AF65 antibody 68166-1-IgDownload protocol
IHC protocol for U2AF2/U2AF65 antibody 68166-1-IgDownload protocol
IF protocol for U2AF2/U2AF65 antibody 68166-1-IgDownload protocol
FC protocol for U2AF2/U2AF65 antibody 68166-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes

Authors - Yanfen Zheng
View Article