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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclonal antibody

U2AF2/U2AF65 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7E7

Cat no : CL488-68166

Synonyms

U2AF65, U2AF2, hU2AF65, hU2AF(65), 1B7E7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclo (CL488-68166)

    IF Staining of HEK-293 using CL488-68166

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 U2AF2 antibody (CL488-68166, Clone: 1B7E7 ) at dilution of 1:100, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated U2AF2/U2AF65 Monoclo (CL488-68166)

    FC experiment of HEK-293 using CL488-68166

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human U2AF2 (CL488-68166, Clone:1B7E7) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68166 targets U2AF2/U2AF65 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen U2AF2/U2AF65 fusion protein Ag8070 相同性解析による交差性が予測される生物種
Full Name U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight 54 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC008740
Gene symbol U2AF2
Gene ID (NCBI) 11338
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Download protocol
FC protocol for CL Plus 488 U2AF2/U2AF65 antibody CL488-68166Download protocol
Standard Protocols
Click here to view our Standard Protocols