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  • Featured Product
  • KD/KO Validated

UAP1 Monoclonal antibody, PBS Only

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C7B2

Cat no : 67545-1-PBS

Synonyms

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UAP1 Monoclonal antibody (67545-1-Ig)

    WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UAP1 Monoclonal antibody (67545-1-Ig)

    WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UAP1 Monoclonal antibody (67545-1-Ig)

    IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UAP1 Monoclonal antibody (67545-1-Ig)

    IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using UAP1 Monoclonal antibody (67545-1-Ig)

    IF Staining of PC-3 using 67545-1-Ig (same clone as 67545-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67545-1-PBS targets UAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UAP1 fusion protein Ag9513 相同性解析による交差性が予測される生物種
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57-59 kDa
GenBank accession numberBC009377
Gene symbol UAP1
Gene ID (NCBI) 6675
RRIDAB_2882762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)