Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, NCI-H1299 cells, LNCaP cells, HepG2 cells, THP-1 cells, HEK-293 cells, DU 145 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
67545-1-Ig targets UAP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | UAP1 fusion protein Ag9513 相同性解析による交差性が予測される生物種 |
| Full Name | UDP-N-acteylglucosamine pyrophosphorylase 1 |
| Calculated molecular weight | 505 aa, 57 kDa |
| Observed molecular weight | 57-59 kDa |
| GenBank accession number | BC009377 |
| Gene Symbol | UAP1 |
| Gene ID (NCBI) | 6675 |
| RRID | AB_2882762 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UAP1 antibody 67545-1-Ig | Download protocol |
| IHC protocol for UAP1 antibody 67545-1-Ig | Download protocol |
| IF protocol for UAP1 antibody 67545-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Mol Cell Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response
| ||
Front Immunol The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma |