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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UAP1 Monoclonal antibody

UAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C7B2

Cat no : CL488-67545

Synonyms

AntigenX, Antigen X, AGX1, AGX 2, AgX

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated UAP1 Monoclonal anti (CL488-67545)

    IF Staining of PC-3 using CL488-67545

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 UAP1 antibody (CL488-67545, Clone: 2C7B2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated UAP1 Monoclonal anti (CL488-67545)

    FC experiment of PC-3 using CL488-67545

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UAP1 (CL488-67545, Clone:2C7B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67545 targets UAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UAP1 fusion protein Ag9513 相同性解析による交差性が予測される生物種
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57-59 kDa
GenBank accession numberBC009377
Gene symbol UAP1
Gene ID (NCBI) 6675
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UAP1 antibody CL488-67545Download protocol
FC protocol for CL Plus 488 UAP1 antibody CL488-67545Download protocol
Standard Protocols
Click here to view our Standard Protocols