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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-12093 targets UBA5 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UBA5 fusion protein Ag2734 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-like modifier activating enzyme 5 |
| Calculated molecular weight | 404 aa, 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC009737 |
| Gene Symbol | UBA5 |
| Gene ID (NCBI) | 79876 |
| RRID | AB_3083917 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZZ9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBA5(Ubiquitin-like modifier-activating enzyme 5) is also named as UBE1DC1,ThiFP1.It has been detected highest UBE1DC1 expression in spleen, high expression in most other tissues examined, and low expression in colon, prostate, brain, and ovary by RT-PCR analysis(PMID:16328888).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 UBA5 antibody CL488-12093 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |