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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis using 11373-1-AP (same clone as 11373-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis of NIH/3T3 using 11373-1-AP (same clone as 11373-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500. . This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    WB analysis of HeLa using 11373-1-AP (same clone as 11373-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    IP experiment of Jurkat using 11373-1-AP (same clone as 11373-1-PBS)

    IP result of anti-UBE2D1/2/3/4 (IP:11373-1-AP, 4ug; Detection:11373-1-AP 1:300) with Jurkat cells lysate 2600 ug. This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using UBE2D1/2/3/4 Polyclonal antibody (11373-1-AP)

    IHC staining of human prostate cancer using 11373-1-AP (same clone as 11373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11373-1-AP (UBE2D1-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11373-1-PBS in a different storage buffer formulation.

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UBE2D1/2/3/4 Polyclonal antibody, PBS Only

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11373-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11373-1-PBS targets UBE2D1/2/3/4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2D1/2/3/4 fusion protein Ag1932 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC015997
Gene Symbol UBE2D1
Gene ID (NCBI) 7321
RRIDAB_2210129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51668
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.