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  • Featured Product
  • KD/KO Validated

UBE2E2 Polyclonal antibody, PBS Only

UBE2E2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11844-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human thyroid gland tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of human thyroid gland using 11844-1-AP (same clone as 11844-1-PBS)

    human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of human brain using 11844-1-AP (same clone as 11844-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse embryo tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of mouse embryo using 11844-1-AP (same clone as 11844-1-PBS)

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    IHC staining of human gliomas using 11844-1-AP (same clone as 11844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11844-1-AP (UBE2E2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBE2E2 Polyclonal antibody (11844-1-AP)

    IF Staining of HepG2 using 11844-1-AP (same clone as 11844-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using UBE2E2 antibody 11844-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11844-1-PBS targets UBE2E2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2E2 fusion protein Ag2509 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC022332
Gene symbol UBE2E2
Gene ID (NCBI) 7325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).