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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated UBXD2 Polyclonal antibody

UBXD2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21052

Synonyms

UBXN4, UBX domain-containing protein 4, UBX domain-containing protein 2, UBX domain protein 4, KIAA2042

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated UBXD2 Polyclonal ant (CL488-21052)

    IF Staining of HEK-293 using CL488-21052

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 UBXD2 antibody (CL488-21052) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated UBXD2 Polyclonal ant (CL488-21052)

    FC experiment of HEK-293 using CL488-21052

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Ubxd2 Polyclonal Antibody (CL488-21052)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-21052 targets UBXD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBXD2 fusion protein Ag13440 相同性解析による交差性が予測される生物種
Full Name UBX domain protein 4
Calculated molecular weight 508 aa, 57 kDa
Observed molecular weight 64-68 kDa
GenBank accession numberBC035594
Gene symbol UBXD2
Gene ID (NCBI) 23190
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 UBXD2 antibody CL488-21052Download protocol
FC protocol for CL Plus 488 UBXD2 antibody CL488-21052Download protocol
Standard Protocols
Click here to view our Standard Protocols