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UCH-L1/PGP9.5 Polyclonal antibody

UCH-L1/PGP9.5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14730-1-AP

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP9/5, PGP 9.5


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Tested Applications

Positive WB detected inA549 cells, NCI-H1299 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inA549 cells, mouse brain tissue
Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14730-1-AP targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, non-human primate
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC000332
Gene symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2210497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protocols

Product Specific Protocols
WB protocol for UCH-L1/PGP9.5 antibody 14730-1-APDownload protocol
IHC protocol for UCH-L1/PGP9.5 antibody 14730-1-APDownload protocol
IF protocol for UCH-L1/PGP9.5 antibody 14730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

The gut metabolite indole-3 propionate promotes nerve regeneration and repair.

Authors - Elisabeth Serger
mouseIF

Nat Biotechnol

Whole-body cellular mapping in mouse using standard IgG antibodies

Authors - Hongcheng Mai
mouseWB

Nature

A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.

Authors - Putianqi Wang
mouseIHC

Cell Metab

The circadian clock time tunes axonal regeneration

Authors - Francesco De Virgiliis
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
mouseIF

Cell Metab

Metabolic stress drives sympathetic neuropathy within the liver.

Authors - Kaili Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaolu (Verified Customer) (06-05-2024)

This antibody works very well on WB. I tried dilution and found that 1:25000 is good enough. Do not reduced the dilution, or you will get a very strong signal.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:25000
  • Cell Tissue Type: Rat cell and Human cell
UCH-L1/PGP9.5 Antibody Western Blot validation (1:25000 dilution) in Rat cell and Human cell (Cat no:14730-1-AP)
FH

Dan (Verified Customer) (10-30-2023)

This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Breast cancer and colorectal cancer cell lines
UCH-L1/PGP9.5 Antibody Western Blot validation (1:2000 dilution) in Breast cancer and colorectal cancer cell lines (Cat no:14730-1-AP)
FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse ovary
UCH-L1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)
FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: paw skin
UCH-L1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)
FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse Brain Homogenate