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- KD/KO Validated
CoraLite®594-conjugated UCH-L1/PGP9.5 Polyclonal antibody
UCH-L1/PGP9.5 Polyclonal Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-14730
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL594-14730 targets UCH-L1/PGP9.5 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UCH-L1/PGP9.5 fusion protein Ag6490 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC000332 |
| Gene symbol | UCHL1 |
| Gene ID (NCBI) | 7345 |
| RRID | AB_2919814 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 UCH-L1/PGP9.5 antibody CL594-14730 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation CX3CR1+/UCHL1+ microglial extracellular vesicles in blood: a potential biomarker for multiple sclerosis |
