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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCH-L1/PGP9.5 Recombinant antibody (84154-5-RR)

    WB analysis using 84154-5-RR (same clone as 84154-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84154-5-RR (UCHL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using UCH-L1/PGP9.5 Recombinant antibody (84154-5-RR)

    IF Staining of mouse brain using 84154-5-RR (same clone as 84154-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-5-RR, Clone: 241107B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84154-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84154-5-RR

    Affinity and Kinetic Characterization of 84154-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84154-5-RR against Human UCH-L1/PGP9.5 were performed. The affinity constant is 6.10 nM.

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UCH-L1/PGP9.5 Recombinant antibody, PBS Only

UCH-L1/PGP9.5 Uni-rAbTM Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 84154-5-PBS
Clone No.241107B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84154-5-PBS targets UCH-L1/PGP9.5 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCH-L1/PGP9.5 fusion protein Ag6490 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Calculated molecular weight 25 kDa
Observed molecular weight27 kDa
GenBank accession numberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09936
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).