Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84154-5-RR targets UCH-L1/PGP9.5 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | UCH-L1/PGP9.5 fusion protein Ag6490 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC000332 |
| Gene Symbol | UCHL1 |
| Gene ID (NCBI) | 7345 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P09936 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UCH-L1/PGP9.5 antibody 84154-5-RR | Download protocol |
| IF protocol for UCH-L1/PGP9.5 antibody 84154-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |