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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCHL3 Recombinant antibody, PBS Only

UCHL3 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242015A10

Cat no : 84542-6-PBS

Synonyms

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UCHL3 Recombinant antibody (84542-6-RR)

    IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84542-6-PBS is UCHL3 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCHL3 fusion protein Ag3053 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC018125
Gene symbol UCHL3
Gene ID (NCBI) 7347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.