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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UCHL3 Recombinant antibody

UCHL3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

242015A10

Cat no : 84542-6-RR

Synonyms

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UCHL3 Recombinant antibody (84542-6-RR)

    WB analysis using 84542-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UCHL3 Recombinant antibody (84542-6-RR)

    IHC staining of mouse testis using 84542-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84542-6-RR

    Affinity and Kinetic Characterization of 84542-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

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Tested Applications

Positive WB detected inHeLa cells, mouse spleen tissue, MCF-7 cells, Jurkat cells, SH-SY5Y cells, BxPC-3 cells, rat spleen tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84542-6-RR targets UCHL3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCHL3 fusion protein Ag3053 相同性解析による交差性が予測される生物種
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC018125
Gene symbol UCHL3
Gene ID (NCBI) 7347
RRIDAB_3672047
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

Protocols

Product Specific Protocols
WB protocol for UCHL3 antibody 84542-6-RRDownload protocol
IHC protocol for UCHL3 antibody 84542-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols