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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis using 12271-1-AP (same clone as 12271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    WB analysis of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    IP experiment of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

    IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug. This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using UCK1 Polyclonal antibody (12271-1-AP)

    IF Staining of MCF-7 using 12271-1-AP (same clone as 12271-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12271-1-PBS in a different storage buffer formulation.

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UCK1 Polyclonal antibody, PBS Only

UCK1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12271-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12271-1-PBS targets UCK1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCK1 fusion protein Ag2912 相同性解析による交差性が予測される生物種
Full Name uridine-cytidine kinase 1
Calculated molecular weight 277 aa, 31 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC015547
Gene Symbol UCK1
Gene ID (NCBI) 83549
RRIDAB_11182173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HA47
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.