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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    WB analysis of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using UCP1 Recombinant antibody (83870-1-RR)

    IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using UCP1 Recombinant antibody (83870-1-RR)

    IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brown adipose tissue using UCP1 Recombinant antibody (83870-1-RR)

    IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83870-1-RR

    Affinity and Kinetic Characterization of 83870-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

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UCP1 Recombinant antibody, PBS Only

UCP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83870-1-PBS
Clone No.240782F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

UCP 1, UCP, Thermogenin, SLC25A7, 240782F3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83870-1-PBS targets UCP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UCP1 fusion protein Ag20388 相同性解析による交差性が予測される生物種
Full Name uncoupling protein 1 (mitochondrial, proton carrier)
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC098168
Gene Symbol UCP1
Gene ID (NCBI) 7350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.